Protein Tyrosine Phosphatases by Rafael Pulido

Author:Rafael Pulido
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

2 Materials

2.1 MEF Cell Culture

1. Primary mouse embryo fibroblasts (MEFs) (see Note 1 ).

2.Dulbecco’s Modified Eagle’s Medium (DMEM) with GlutaMAX supplement, pyruvate, and 4.5 g/l d+glucose (Invitrogen).

3.Clear bottomed, black-walled 96-well plates (Corning Costar plate 3904).

4.Fetal bovine serum (FBS).

5.Trypsin (0.25 %) and ethylenediamine tetraacetic acid (EDTA) mix dissolved in phosphate buffered saline (PBS).

6.PBS: 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4, and 1.47 mM KH2PO4, pH 7.4.

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